Journal: Brain and Behavior
Article Title: Melatonin mitigates type 1 diabetes‐aggravated cerebral ischemia‐reperfusion injury through anti‐inflammatory and anti‐apoptotic effects
doi: 10.1002/brb3.3118
Figure Lengend Snippet: Effects of a single dose of melatonin (10 mg/kg given at 30 min before ischemia) on type 1 diabetes mellitus (T1DM)‐aggravated inflammatory and apoptosis markers using immunofluorescence staining at 24 h of cerebral ischemia–reperfusion injury (CIRI) achieved by 75‐min middle cerebral artery occlusion (MCAO). (a) Representative fluorescence photomicrographs of iNOS+ cells (scale bar = 50 μm). (b) Relative iNOS+ cell density in vehicle ( n = 6) and melatonin ( n = 5) groups. (c) Representative fluorescence photomicrographs of cluster of differentiation (CD)‐68+ cells (scale bar = 100 μm). (d) Relative CD‐68+ cell density in vehicle ( n = 6) and melatonin ( n = 5) groups. (e) Representative fluorescence photomicrographs of TUNEL+ apoptotic cells (scale bar = 50 μm). (f) Relative TUNEL+ cell density in vehicle ( n = 4) and melatonin ( n = 5) groups. (g) Representative fluorescence photomicrographs of neuN+ surviving neurons (scale bar = 50 μm). (h) Relative NeuN+ cell density in vehicle ( n = 6) and melatonin ( n = 5) groups. * p < .05 and ** p < .01 between groups using Student's t ‐test.
Article Snippet: Primary antibody against CD‐68 (1:500) was purchased from AbD Serotec (Oxford, United Kingdom).
Techniques: Immunofluorescence, Staining, Fluorescence, TUNEL Assay